8EI7
Crystal structure of the WWP2 HECT domain in complex with H304, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.160, 64.300, 103.690 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 40.120 - 2.220 |
| R-factor | 0.2031 |
| Rwork | 0.200 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y07 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.690 | 2.290 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.075 | 0.709 |
| Number of reflections | 40576 | 3697 |
| <I/σ(I)> | 13.1 | 2.3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.1 | 5.1 |
| CC(1/2) | 0.998 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2M magnesium chloride, 0.1M Bis-tris pH7.5, 30% PEG3350 |
