8EI5
Crystal structure of the WWP2 HECT domain in complex with H301, a Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.660, 63.100, 122.260 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 45.400 - 2.600 |
| R-factor | 0.2155 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y07 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.680 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.139 | 0.616 |
| Number of reflections | 56697 | 4596 |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.1 | 5.2 |
| CC(1/2) | 0.995 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1M Sodium acetate, 0.1M sodium citrate pH6.0, 10% PEG4000 |
