8EHN
PRRSV-1 PLP2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-09-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97934 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 67.499, 102.347, 100.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.580 - 2.300 |
R-factor | 0.20534 |
Rwork | 0.200 |
R-free | 0.25296 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.440 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.580 | 8.910 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.042 | |
Number of reflections | 15677 | 1516 |
<I/σ(I)> | 16.7 | |
Completeness [%] | 99.1 | |
Redundancy | 4.1 | |
CC(1/2) | 0.998 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 22% PEG 2000, 0.35M ammonium acetate, 0.1M HEPES 7.5 |