8EGW

Complex of Fat4(EC1-4) bound to Dchs1(EC1-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-11
Detector	RAYONIX MX-300
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	88.660, 61.370, 100.966
Unit cell angles	90.00, 112.74, 90.00

Refinement procedure

Resolution	49.083 - 2.300
R-factor	0.192
Rwork	0.190
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a4c 4zpl 4zi8 4zpo 5iu9 3q2w
RMSD bond length	0.009
RMSD bond angle	1.041
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.085	2.283
High resolution limit [Å]	2.204	2.204
Number of reflections	50558	5002
<I/σ(I)>	8.87
Completeness [%]	99.3	98.24
Redundancy	3.4
CC(1/2)	0.995	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.08 M Magnesium Chloride; 0.1 M Sodium Cacodylate, pH 6.1; 20% Polyethylene Glycol 1000