8EFG
Crystal structure of human TATDN1 bound to dAMP and two zinc ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.127230 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.880, 76.510, 77.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.300 - 1.500 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xio |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.542 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.505 |
| Rmeas | 0.060 | 0.743 |
| Number of reflections | 52962 | 8656 |
| <I/σ(I)> | 15.93 | 3.02 |
| Completeness [%] | 99.4 | 92.7 |
| Redundancy | 6.62 | 6.7 |
| CC(1/2) | 0.999 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 19% PEG 8000, 100 mM HEPES pH 7.0 |
