8EEC
Crystal structure of HPK1 citron-homology domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-07-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 81.667, 81.667, 140.535 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.614 - 2.500 |
| R-factor | 0.2108 |
| Rwork | 0.208 |
| R-free | 0.25670 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.8) |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.850 | 46.850 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.156 | 0.039 | 1.390 |
| Rmeas | 0.163 | 0.041 | 1.446 |
| Rpim | 0.045 | 0.013 | 0.395 |
| Total number of observations | 218660 | 6822 | 32537 |
| Number of reflections | 17174 | 649 | 2449 |
| <I/σ(I)> | 18.6 | 57.7 | 2.1 |
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 12.7 | 10.5 | 13.3 |
| CC(1/2) | 0.998 | 0.999 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.4 M ammonium phosphate |
