8EE8

Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ100-C in complex with ZIKV E glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	101.840, 126.680, 140.480
Unit cell angles	90.00, 89.97, 90.00

Refinement procedure

Resolution	14.984 - 2.803
R-factor	0.2457
Rwork	0.241
R-free	0.29550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nip
RMSD bond length	0.003
RMSD bond angle	0.743
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.984	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	62002	6273
<I/σ(I)>	11.7
Completeness [%]	70.8
Redundancy	2.6
CC(1/2)	0.880	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	12% PEG 8000, 0.2 M (NH4)2SO4, 0.1M Tris (pH 8.5)