8EE5
Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ119-D in complex with ZIKV E glycoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 51.681, 103.672, 196.698 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.994 - 3.583 |
R-factor | 0.2513 |
Rwork | 0.249 |
R-free | 0.29640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6nip |
RMSD bond length | 0.003 |
RMSD bond angle | 0.680 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 3.720 |
High resolution limit [Å] | 3.580 | 3.580 |
Rmeas | 0.210 | |
Number of reflections | 23586 | 1900 |
<I/σ(I)> | 9.97 | |
Completeness [%] | 98.6 | |
Redundancy | 6.6 | |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.06M Nitrate Phosphate Sulfate , 0.1M Sodium HEPES and MOPS (acid) pH 7.5, 20% Ethylene glycol, 10 % PEG 8000 + 2% w/v Benzamidine hydrochloride |