8EDQ
E. coli pyruvate kinase (PykF) I264F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-10-23 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 74.870, 244.931, 134.284 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.240 - 2.880 |
R-factor | 0.2058 |
Rwork | 0.202 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yng |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.240 | 2.983 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmerge | 0.078 | 0.343 |
Number of reflections | 28406 | 2804 |
<I/σ(I)> | 13.05 | 2.66 |
Completeness [%] | 99.8 | 99.43 |
Redundancy | 1.9 | |
CC(1/2) | 0.990 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Sodium formate, 0.1 M Bis-Tris propane, 20 % w/v PEG 3350 |