8EDJ
Crystal structure of rA3G-ssRNA-GA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.305, 68.058, 129.312 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.027 - 1.830 |
R-factor | 0.1689 |
Rwork | 0.168 |
R-free | 0.19210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7uu4 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.085 | |
Number of reflections | 83087 | 4301 |
<I/σ(I)> | 21.01 | |
Completeness [%] | 99.9 | |
Redundancy | 12.7 | |
CC(1/2) | 0.999 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.18 M Na2SO4, 20% PEG 3350 |