8ED4

Structure of the complex between the arsenite oxidase and its native electron acceptor cytochrome c552 from Pseudorhizobium sp. str. NT-26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	P 1 21 1
Unit cell lengths	129.400, 126.560, 148.020
Unit cell angles	90.00, 107.81, 90.00

Refinement procedure

Resolution	49.230 - 2.250
R-factor	0.1835
Rwork	0.181
R-free	0.23015
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4aay 1co6
RMSD bond length	0.007
RMSD bond angle	1.606
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.230	2.290
High resolution limit [Å]	2.250	2.250
Number of reflections	214836	10599
<I/σ(I)>	11.4
Completeness [%]	99.9
Redundancy	7
CC(1/2)	0.998	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M sodium chloride, 0.1 M HEPES pH 7.3, 18% (w/v) PEG 3,350