8EBL

Structure of KLHDC2 substrate binding domain bound to C-degron from EPHB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	49.480, 84.482, 90.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.440 - 1.370
R-factor	0.1502
Rwork	0.149
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6do2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.420
High resolution limit [Å]	1.370	2.950	1.370
Rmerge	0.039	0.033	0.617
Rmeas	0.043	0.037	0.672
Rpim	0.018	0.015	0.264
Total number of observations	906211
Number of reflections	149088	15505	14577
<I/σ(I)>	10.3
Completeness [%]	95.8	98.2	93.8
Redundancy	6.1	6	6.3
CC(1/2)		0.989	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	10-15% PEG3350, 0.2M NaSCN, 0.1 M Bis-Tris Propane, pH = 7.5