8E8T
Structure of the short LOR domain of human AASS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.92010 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 77.356, 154.715, 70.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.910 - 2.180 |
R-factor | 0.2152 |
Rwork | 0.214 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qrj |
RMSD bond length | 0.003 |
RMSD bond angle | 0.560 |
Data reduction software | autoPROC |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.910 | 42.910 | 2.250 |
High resolution limit [Å] | 2.180 | 8.990 | 2.180 |
Rmerge | 0.172 | 0.079 | 1.089 |
Rmeas | 0.206 | 0.096 | 1.319 |
Rpim | 0.110 | 0.052 | 0.724 |
Total number of observations | 132605 | 1976 | 10951 |
Number of reflections | 43284 | 645 | 3757 |
<I/σ(I)> | 4.4 | 11.8 | 1 |
Completeness [%] | 96.7 | 90.4 | 98 |
Redundancy | 3.1 | 3.1 | 2.9 |
CC(1/2) | 0.981 | 0.984 | 0.367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 0.2M NaCl, 0.1M HEPES, 25% PEG 4000 |