8E7O
CRYSTAL STRUCTURE OF LYS48-LINKED TETRAUBIQUITIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.839, 77.164, 135.823 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.911 - 1.700 |
| Rwork | 0.157 |
| R-free | 0.22060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aar |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.190 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.911 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 34404 | 1754 |
| <I/σ(I)> | 1.27 | |
| Completeness [%] | 99.3 | 96.8 |
| Redundancy | 7.8 | 7.3 |
| CC(1/2) | 1.000 | 0.355 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 25% w/v PEG 3350, 0.2M ammonium sulfate, 0.1M Bis-Tris pH 5.5 |
