8E67
ETV6 H396Y variant bound to DNA containing the sequence GGAT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.521270 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.940, 74.350, 76.620 |
Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
Resolution | 48.200 - 2.300 |
R-factor | 0.214 |
Rwork | 0.211 |
R-free | 0.26670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mhg |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.200 | 48.200 | 2.420 |
High resolution limit [Å] | 2.300 | 5.000 | 2.300 |
Rmerge | 0.062 | 0.035 | 0.683 |
Rmeas | 0.074 | 0.042 | 0.821 |
Number of reflections | 58090 | 3142 | 4449 |
<I/σ(I)> | 10.26 | 25.53 | 1.68 |
Completeness [%] | 99.3 | 98.9 | 99.9 |
Redundancy | 3.287 | 3.235 | 3.208 |
CC(1/2) | 0.998 | 0.998 | 0.748 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Protein in 50 mM NaCl, 20 mM HEPES, pH 7.5 at ~300 uM incubated 1:1 with 50 mM Sodium cacodylate, 25 mM Ammonium acetate, 10 mM MgCl2, 20% PEG-8000, pH 6.0 |