8E67

ETV6 H396Y variant bound to DNA containing the sequence GGAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.521270
Spacegroup name	P 1 21 1
Unit cell lengths	63.940, 74.350, 76.620
Unit cell angles	90.00, 98.02, 90.00

Refinement procedure

Resolution	48.200 - 2.300
R-factor	0.214
Rwork	0.211
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mhg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.200	48.200	2.420
High resolution limit [Å]	2.300	5.000	2.300
Rmerge	0.062	0.035	0.683
Rmeas	0.074	0.042	0.821
Number of reflections	58090	3142	4449
<I/σ(I)>	10.26	25.53	1.68
Completeness [%]	99.3	98.9	99.9
Redundancy	3.287	3.235	3.208
CC(1/2)	0.998	0.998	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Protein in 50 mM NaCl, 20 mM HEPES, pH 7.5 at ~300 uM incubated 1:1 with 50 mM Sodium cacodylate, 25 mM Ammonium acetate, 10 mM MgCl2, 20% PEG-8000, pH 6.0