8E66

ETV6 H396Y variant bound to DNA containing the sequence GGAA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2021-03-11
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	29.841, 94.685, 76.259
Unit cell angles	90.00, 94.76, 90.00

Refinement procedure

Resolution	25.920 - 2.350
R-factor	0.2203
Rwork	0.216
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mhg
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.150	0.055	0.675
Rmeas	0.163	0.059	0.743
Rpim	0.061	0.022	0.304
Total number of observations	115206
Number of reflections	17254	899	810
<I/σ(I)>	4.5
Completeness [%]	98.0	98.6	93.4
Redundancy	6.7	7.4	5.4
CC(1/2)		0.992	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Protein in 50 mM NaCl, 20 mM HEPES, pH 7.5 at ~300 uM incubated 1:1 with 50 mM Sodium cacodylate, 25 mM Ammonium acetate, 10 mM MgCl2, 20% PEG-8000, pH 6.0