8E51

Crystal Structure of Iridescent Shark Catfish Cadherin-23 EC1-2 and Protocadherin-15 EC1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-19
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97934
Spacegroup name	P 32 2 1
Unit cell lengths	75.816, 75.816, 242.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.599 - 2.594
Rwork	0.202
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AlphaFold2 Model
RMSD bond length	0.008
RMSD bond angle	1.526
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.599	2.640
High resolution limit [Å]	2.594	2.600
Number of reflections	26033	1264
<I/σ(I)>	12.907	6.53
Completeness [%]	100.0	99.8
Redundancy	28.1	20.7
CC(1/2)	0.975	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M CaCl2, 0.1 M HEPES pH 7.5, 30% PEG 4000