8E51
Crystal Structure of Iridescent Shark Catfish Cadherin-23 EC1-2 and Protocadherin-15 EC1-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-19 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97934 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 75.816, 75.816, 242.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.599 - 2.594 |
Rwork | 0.202 |
R-free | 0.24240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold2 Model |
RMSD bond length | 0.008 |
RMSD bond angle | 1.526 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.599 | 2.640 |
High resolution limit [Å] | 2.594 | 2.600 |
Number of reflections | 26033 | 1264 |
<I/σ(I)> | 12.907 | 6.53 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 28.1 | 20.7 |
CC(1/2) | 0.975 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M CaCl2, 0.1 M HEPES pH 7.5, 30% PEG 4000 |