8E2P
Crystal structure of TadA*8.20 in a complex with ssDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97932 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.670, 84.670, 214.930 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.340 - 2.720 |
R-factor | 0.197 |
Rwork | 0.190 |
R-free | 0.21800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z3a |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.340 | 2.790 |
High resolution limit [Å] | 2.720 | 2.720 |
Number of reflections | 24824 | 1776 |
<I/σ(I)> | 9.4 | |
Completeness [%] | 100.0 | |
Redundancy | 10.9 | |
CC(1/2) | 0.995 | 0.389 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3,350, ammonium acetate, TRIS pH 8.5 |