8E0D
Crystal structure of human Sar1bE140D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-05-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.828, 38.420, 55.607 |
| Unit cell angles | 90.00, 101.63, 90.00 |
Refinement procedure
| Resolution | 44.481 - 1.981 |
| R-factor | 0.1611 |
| Rwork | 0.157 |
| R-free | 0.20030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8dzm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.122 | 0.273 |
| Rmeas | 0.122 | 0.304 |
| Rpim | 0.047 | 0.121 |
| Number of reflections | 13267 | 644 |
| <I/σ(I)> | 22 | 5.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.3 |
| CC(1/2) | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.9M NaCl, 0.2M CaCl2 and 0.1M NaAc, pH4.5. |
