8E0C
Crystal structure of human Sar1bH79G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9778 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.652, 52.525, 81.405 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.148 - 1.993 |
R-factor | 0.1761 |
Rwork | 0.170 |
R-free | 0.23390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dzm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.266 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.993 | 1.993 |
Rmerge | 0.164 | 0.438 |
Number of reflections | 12363 | 601 |
<I/σ(I)> | 21.6 | 7.8 |
Completeness [%] | 99.5 | 97.4 |
Redundancy | 7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2M (NH4)2SO4 and 0.1M sodium citrate, pH5.5. |