8E0C

Crystal structure of human Sar1bH79G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9778
Spacegroup name	P 21 21 21
Unit cell lengths	40.652, 52.525, 81.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.148 - 1.993
R-factor	0.1761
Rwork	0.170
R-free	0.23390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8dzm
RMSD bond length	0.008
RMSD bond angle	1.266
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.030
High resolution limit [Å]	1.993	1.993
Rmerge	0.164	0.438
Number of reflections	12363	601
<I/σ(I)>	21.6	7.8
Completeness [%]	99.5	97.4
Redundancy	7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2M (NH4)2SO4 and 0.1M sodium citrate, pH5.5.