8E0B
Crystal structure of human Sar1bT39N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.971, 61.814, 70.681 |
| Unit cell angles | 90.00, 106.17, 90.00 |
Refinement procedure
| Resolution | 36.166 - 2.212 |
| R-factor | 0.1798 |
| Rwork | 0.174 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8dzm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.117 | 0.492 |
| Number of reflections | 20483 | 745 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 93.4 | 70.7 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2M (NH4)2SO4 and 0.1M sodium citrate, pH5.5. |
