8E0A
Crystal structure of human Sar1b
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.966, 61.485, 70.589 |
| Unit cell angles | 90.00, 107.34, 90.00 |
Refinement procedure
| Resolution | 29.545 - 1.797 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8dzm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.073 | 0.303 |
| Number of reflections | 40321 | 2011 |
| <I/σ(I)> | 32.5 | 5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.2M (NH4)2SO4 and 0.1M sodium citrate, pH5.5. |
