8DZO
Crystal structure of human Sar1T39N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9778 |
Spacegroup name | P 61 |
Unit cell lengths | 55.605, 55.605, 222.404 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.401 - 1.800 |
R-factor | 0.1613 |
Rwork | 0.160 |
R-free | 0.19320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dzm |
RMSD bond length | 0.006 |
RMSD bond angle | 0.920 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.263 |
Number of reflections | 35856 | 1756 |
<I/σ(I)> | 36.64 | 12.16 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 10.9 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG3350 (w/v), 0.1M CaCl2, 0.1M Mes-HCl buffer, pH6.0 |