8DZM
Crystal structure of human Sar1a in complex with ppGpp and Magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9778 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.125, 35.933, 76.118 |
Unit cell angles | 90.00, 101.52, 90.00 |
Refinement procedure
Resolution | 45.415 - 1.648 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f6b |
RMSD bond length | 0.007 |
RMSD bond angle | 0.956 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.640 | 1.648 |
Rmeas | 0.085 | 0.683 |
Number of reflections | 43805 | 2066 |
<I/σ(I)> | 18.9 | 1.15 |
Completeness [%] | 99.5 | 95.3 |
Redundancy | 3.4 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12% PEG6000 (w/v), 1.0M LiCl, 0.1M MgCl2, 0.1M sodium acetate buffer, pH4.5 |