8DY8

Crystal structure of the R178Q mutant of ubiquitin carboxy terminal hydrolase L1 (UCH-L1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 4 21 2
Unit cell lengths	109.385, 109.385, 79.202
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.920 - 2.100
R-factor	0.2249
Rwork	0.222
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2etl
RMSD bond length	0.002
RMSD bond angle	0.526
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.064	0.540
Rpim	0.020	0.440
Number of reflections	26476	1559
<I/σ(I)>	18.29	0.76
Completeness [%]	92.4	55.28
Redundancy	9.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	2.4 M Ammonium Sulfate, 0.1 M HEPES