8DTU

The complex of nanobody 5344N74D with BCL11A ZF6.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 43 21 2
Unit cell lengths	57.507, 57.507, 157.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.660 - 2.447
R-factor	0.2639
Rwork	0.261
R-free	0.28760
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.318
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.664	2.535
High resolution limit [Å]	2.447	2.447
Rmerge	0.155	2.123
Rmeas	0.157	2.152
Rpim	0.026	0.351
Number of reflections	10390	1011
<I/σ(I)>	24.49	2.77
Completeness [%]	96.6	90.83
Redundancy	35	36.2
CC(1/2)	0.999	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281.15	0.1 M HEPES, pH 7.5-8.2, 2.2M Li2SO4.