8DS8
Crystal structure of human TNRC18 BAH domain in complex with H3K9me3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 73.804, 120.828, 46.632 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.480 - 1.840 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dov |
RMSD bond length | 0.009 |
RMSD bond angle | 0.974 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.840 | 3.990 | 1.840 |
Rmerge | 0.124 | 0.053 | 1.210 |
Rmeas | 0.139 | 0.059 | 1.380 |
Rpim | 0.061 | 0.025 | 0.649 |
Number of reflections | 36692 | 3909 | 3584 |
<I/σ(I)> | 5.2 | ||
Completeness [%] | 99.5 | 99.2 | 99.7 |
Redundancy | 5.2 | 5.5 | 4.5 |
CC(1/2) | 0.997 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M Sodium Cacodylate, pH 6.5, 0.2 M Magnesium Acetate, 16-20% PEG 8000 |