8DON
Beta-lactamase CTX-M-14 T215A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 41.430, 41.430, 231.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.455 - 1.360 |
R-factor | 0.1457 |
Rwork | 0.145 |
R-free | 0.15950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylt |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.455 | 1.409 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.039 | 0.069 |
Rmeas | 0.056 | 0.098 |
Number of reflections | 50984 | 5041 |
<I/σ(I)> | 11.76 | 7.92 |
Completeness [%] | 99.6 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Calcium chloride ,0.1 M HEPES pH 7, 20% (w/v) PEG 6000 |