8DOE
Crystal Structure of CTX-M-14 N106A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.840, 37.910, 65.460 |
Unit cell angles | 90.00, 103.44, 90.00 |
Refinement procedure
Resolution | 48.480 - 1.500 |
R-factor | 0.1456 |
Rwork | 0.145 |
R-free | 0.16520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylt |
RMSD bond length | 0.010 |
RMSD bond angle | 1.226 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.480 | 1.554 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.054 | 0.272 |
Rmeas | 0.077 | 0.384 |
Number of reflections | 37847 | 3704 |
<I/σ(I)> | 9.12 | |
Completeness [%] | 98.5 | 97.22 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Magnesium chloride ,0.1 M Tris pH 8 20% (w/v), PEG 6000 |