8DO6
The structure of S. epidermidis Cas10-Csm bound to target RNA
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.001 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.1 |
| Number of Particle | 1400000 |
| Software | cryoSPARC,Leginon |
| CTF correction | PHASE FLIPPING ONLY |
