8DN1
Q108K:K40L:T51C:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V at pH 7.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-25 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.1272 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 29.222, 66.833, 63.704 |
Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
Resolution | 29.590 - 1.320 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.21740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qzt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.156 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.590 | 1.369 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmeas | 0.087 | 0.376 |
Rpim | 0.024 | 0.134 |
Number of reflections | 28092 | 2805 |
<I/σ(I)> | 30.8 | |
Completeness [%] | 98.1 | |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 25% PEG 4000, ammonium acetate, sodium acetate pH 4.0 - 4.8 |