8DKT

Crystal Structure of Septin1 - Septin2 heterocomplex from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-26
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	73.577, 149.423, 155.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.560 - 2.380
R-factor	0.2188
Rwork	0.217
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6upq
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.560	2.747
High resolution limit [Å]	2.380	2.380
Rmerge	0.144	1.548
Rmeas	0.159	1.616
Rpim	0.041	0.459
Number of reflections	15075	9267
<I/σ(I)>	11.1	1.7
Completeness [%]	43.2	6.2
Redundancy	13.4	12.3
CC(1/2)	1.000	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	10% w/v PEG 8000, 20% v/v ethylene glycol; 0.1 M MES/imidazole pH 6.5; 0.02 M of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium l-tartrate, sodium oxamate