8DKR
Pseudomonas-phage E217 TerL nuclease domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.601, 62.628, 149.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.930 - 2.050 |
| R-factor | 0.2185 |
| Rwork | 0.217 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c12 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rpim | 0.036 | 0.332 |
| Number of reflections | 31650 | 2226 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 88.1 | 63 |
| Redundancy | 5.8 | 2 |
| CC(1/2) | 0.984 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 0.7 M sodium citrate tribasic dihydrate, 10 mM 2-mercaptoethanol |
