8DIV
Crystal structure of NavAb I22V as a basis for the human Nav1.7 Inherited Erythromelalgia I136V mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.578, 124.578, 190.927 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.970 - 2.540 |
R-factor | 0.2013 |
Rwork | 0.199 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rvy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.071 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.156 | 0.844 |
Rpim | 0.045 | 0.360 |
Number of reflections | 22384 | 415 |
<I/σ(I)> | 13.3 | 0.43 |
Completeness [%] | 87.6 | 33.3 |
Redundancy | 11.5 | 3.6 |
CC(1/2) | 1.000 | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 1.7-1.8 M Ammonium sulfate 0.1 M Sodium Citrate pH 5.0 |