8DHY

N-terminal fragment of MsbA fused to GFP in complex with copper(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-07
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.3782
Spacegroup name	I 21 21 21
Unit cell lengths	70.610, 108.540, 140.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.080 - 2.150
R-factor	0.2029
Rwork	0.202
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.012
RMSD bond angle	1.168
Data reduction software	XDS (Jan 10, 2022)
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	2.210
High resolution limit [Å]	2.150	2.150
Rmeas	0.094	1.450
Number of reflections	56321	4109
<I/σ(I)>	12.35	1.61
Completeness [%]	99.3	98
Redundancy	6.7	6.8
CC(1/2)	0.997	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	70% Tacsimate pH 7.0