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8DH5

T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 5.0.3
Synchrotron siteALS
Beamline5.0.3
Temperature [K]100
Detector technologyCCD
Collection date2022-04-21
DetectorADSC QUANTUM 315r
Wavelength(s)1
Spacegroup nameP 1
Unit cell lengths78.544, 86.305, 201.199
Unit cell angles89.79, 85.39, 69.49
Refinement procedure
Resolution47.430 - 2.850
R-factor0.2341
Rwork0.234
R-free0.26720
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)8dh0
Data reduction softwareXDS
Data scaling softwareAimless (0.7.4)
Phasing softwarePHENIX (1.19)
Refinement softwarePHENIX (1.19)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]47.43047.4302.880
High resolution limit [Å]2.83015.5002.830
Rmerge0.1180.0271.789
Rmeas0.1390.0322.149
Rpim0.0730.0171.169
Total number of observations408951245719213
Number of reflections1158896795736
<I/σ(I)>6.128.10.5
Completeness [%]98.595.898
Redundancy3.53.63.3
CC(1/2)0.9970.9980.297
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.129810% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1

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PDB entries from 2024-11-13

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