8DH5
T7 RNA polymerase elongation complex with unnatural base dPa-ATP mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 78.544, 86.305, 201.199 |
Unit cell angles | 89.79, 85.39, 69.49 |
Refinement procedure
Resolution | 47.430 - 2.850 |
R-factor | 0.2341 |
Rwork | 0.234 |
R-free | 0.26720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dh0 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.19) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.430 | 47.430 | 2.880 |
High resolution limit [Å] | 2.830 | 15.500 | 2.830 |
Rmerge | 0.118 | 0.027 | 1.789 |
Rmeas | 0.139 | 0.032 | 2.149 |
Rpim | 0.073 | 0.017 | 1.169 |
Total number of observations | 408951 | 2457 | 19213 |
Number of reflections | 115889 | 679 | 5736 |
<I/σ(I)> | 6.1 | 28.1 | 0.5 |
Completeness [%] | 98.5 | 95.8 | 98 |
Redundancy | 3.5 | 3.6 | 3.3 |
CC(1/2) | 0.997 | 0.998 | 0.297 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1 |