8DH3
T7 RNA polymerase elongation complex with unnatural base dPa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-28 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 79.067, 86.271, 201.967 |
Unit cell angles | 89.70, 86.01, 69.45 |
Refinement procedure
Resolution | 47.980 - 3.000 |
R-factor | 0.2477 |
Rwork | 0.247 |
R-free | 0.27550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dh0 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.19) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.980 | 47.980 | 2.980 |
High resolution limit [Å] | 2.930 | 16.050 | 2.930 |
Rmerge | 0.114 | 0.026 | 2.204 |
Rmeas | 0.134 | 0.030 | 2.580 |
Rpim | 0.071 | 0.016 | 1.330 |
Total number of observations | 371631 | 2296 | 19142 |
Number of reflections | 105382 | 615 | 5176 |
<I/σ(I)> | 5.9 | 28.1 | 0.4 |
Completeness [%] | 98.4 | 94 | 98.1 |
Redundancy | 3.5 | 3.7 | 3.7 |
CC(1/2) | 0.998 | 0.999 | 0.306 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1 |