8DH2
T7 RNA polymerase elongation complex with unnatural base dDs-ATP mismatch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-04-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 78.702, 85.047, 200.477 |
Unit cell angles | 89.87, 85.38, 69.51 |
Refinement procedure
Resolution | 47.370 - 2.900 |
R-factor | 0.2609 |
Rwork | 0.260 |
R-free | 0.30750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dh0 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX (1.19) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.550 | 48.550 | 2.940 |
High resolution limit [Å] | 2.890 | 15.830 | 2.890 |
Rmerge | 0.139 | 0.022 | 2.003 |
Rmeas | 0.164 | 0.025 | 2.370 |
Rpim | 0.087 | 0.013 | 1.251 |
Total number of observations | 376957 | 2336 | 18952 |
Number of reflections | 106891 | 632 | 5354 |
<I/σ(I)> | 5.5 | 31.8 | 0.5 |
Completeness [%] | 98.3 | 95.5 | 98 |
Redundancy | 3.5 | 3.7 | 3.5 |
CC(1/2) | 0.995 | 0.999 | 0.273 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 298 | 10% PEG 8000, 8% glycerol, 5 mM B-mercaptoethanol, 100 mM Tris pH 8.1 |