8DC7
Crystal structure of p53 Y220C covalently bound to indole KG10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2021-12-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 68.660, 85.920, 84.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.252 - 1.987 |
R-factor | 0.161901558302 |
Rwork | 0.160 |
R-free | 0.19296 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j1x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.027 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.020 | 1.990 |
High resolution limit [Å] | 1.980 | 1.990 |
Number of reflections | 17477 | 1722 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 100.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM HEPES, 2.2M MgSO4 |