8DC0

Rat Betaglycan Zona Pellucida Domain (ZPC) in complex with mini monomer TGFb2 (mmTGF-b2-7M2R)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	76.456, 90.453, 98.872
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.740 - 1.930
R-factor	0.2215
Rwork	0.219
R-free	0.25660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3QW9 & 5TX4
RMSD bond length	0.006
RMSD bond angle	0.915
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.740	1.999
High resolution limit [Å]	1.930	1.930
Rmerge	0.179
Rmeas	0.186
Number of reflections	26131	2560
<I/σ(I)>	9.27
Completeness [%]	99.8
Redundancy	13
CC(1/2)	0.997	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	13% PEG4000, 0.1 M trisodium citrate, 10% ethylene glycol