8DBJ
Human PRPS1 with Phosphate, ATP, and R5P; Filament Interface
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2 |
| Software | RELION,PHENIX,Leginon,CTFFIND,Coot,cryoSPARC |
| Symmetry | D3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
