8DAP
[GA/TC] Self-Assembled 3D DNA Tensegrity Triangle with 24 bp Arm Length forming a Trigonal Hexagon
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-12 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.00743 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 166.609, 166.609, 119.318 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.130 - 6.470 |
R-factor | 0.142 |
Rwork | 0.140 |
R-free | 0.17340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gbi |
RMSD bond length | 0.008 |
RMSD bond angle | 1.111 |
Data reduction software | autoPROC |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.140 | 6.820 |
High resolution limit [Å] | 6.470 | 6.470 |
Number of reflections | 3631 | 203 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 91.1 | |
Redundancy | 19.7 | |
CC(1/2) | 0.999 | 0.283 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 1.75 M ammonium sulfate, 120 mM Tris, 120 mM Acetic Acid, 6 mM EDTA |