8DAB
Coevolved affibody-Z domain pair LL2.c17
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.77488 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.091, 35.532, 49.433 |
Unit cell angles | 90.00, 115.42, 90.00 |
Refinement procedure
Resolution | 31.650 - 1.134 |
R-factor | 0.1738 |
Rwork | 0.172 |
R-free | 0.19950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5djt |
RMSD bond length | 0.007 |
RMSD bond angle | 0.828 |
Data reduction software | DIALS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.650 | 1.175 |
High resolution limit [Å] | 1.134 | 1.134 |
Rmerge | 0.092 | 4.141 |
Rmeas | 0.097 | 4.383 |
Rpim | 0.030 | 1.415 |
Number of reflections | 27318 | 140 |
<I/σ(I)> | 15.86 | 1.21 |
Completeness [%] | 60.8 | 3.1 |
Redundancy | 9.8 | 9.4 |
CC(1/2) | 0.999 | 0.321 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 295 | 2.3 M Amm2HPO4, 100 mM Tris pH 8.2 cryoprotected in 30% glycerol |