8DA7
Coevolved affibody-Z domain pair LL1.c6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-18 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.77487 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.617, 42.380, 74.222 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.270 - 1.020 |
R-factor | 0.1612 |
Rwork | 0.160 |
R-free | 0.18280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5djt |
RMSD bond length | 0.016 |
RMSD bond angle | 1.420 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.910 | 1.056 |
High resolution limit [Å] | 1.020 | 1.020 |
Rmerge | 0.065 | 2.470 |
Rmeas | 0.069 | 2.631 |
Rpim | 0.024 | 0.895 |
Number of reflections | 57264 | 5584 |
<I/σ(I)> | 12.91 | 0.8 |
Completeness [%] | 98.8 | 97.38 |
Redundancy | 8.1 | 8.4 |
CC(1/2) | 0.999 | 0.456 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 2.5 M AmmSO4, 100 mM tris pH 8.5 cryoprotected with Na malonate |