8D5R
Structure of Y430F D-ornithine/D-lysine decarboxylase complex with D-ornithine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.530, 50.200, 140.280 |
Unit cell angles | 90.00, 116.00, 90.00 |
Refinement procedure
Resolution | 37.340 - 1.440 |
R-factor | 0.165 |
Rwork | 0.165 |
R-free | 0.19030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6N2H.pdb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.071 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.340 | 1.491 |
High resolution limit [Å] | 1.440 | 1.440 |
Number of reflections | 160330 | 15778 |
<I/σ(I)> | 12.7 | 0.79 |
Completeness [%] | 99.8 | |
Redundancy | 20 | |
CC(1/2) | 0.998 | 0.374 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES, pH 7.5, 0.2 M NaOAc, 20% PEG 4000 |