8D5P
Mouse TCR TG6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-21 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.982 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.160, 196.331, 63.112 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.238 - 2.750 |
| Rwork | 0.224 |
| R-free | 0.30060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3to4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.000 | 2.910 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rpim | 0.010 | 0.100 |
| Number of reflections | 28636 | 28062 |
| <I/σ(I)> | 16 | |
| Completeness [%] | 98.5 | |
| Redundancy | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% w/v PEG10K, 0.1M Sodium citrate tribasic dihydrate pH 5.5, 1M Lithium sulfate monohydrate |
