3TO4

Structure of mouse Valpha14Vbeta2-mouseCD1d-alpha-Galactosylceramide

Summary for 3TO4

• Entry DOI: 10.2210/pdb3to4/pdb
• Related: 3HE6 3HE7
• Descriptor: Antigen-presenting glycoprotein CD1d1, Beta-2 microglobulin, NKT Valpha14 (MOUSE VARIABLE DOMAIN, HUMAN CONSTANT DOMAIN), ... (8 entities in total)
• Functional Keywords: mouse cd1d, mouse nkt, immune system
• Biological source: Mus musculus (mouse); More
• Total number of polymer chains: 4
• Total formula weight: 100178.40

Authors

Patel, O.,Rossjohn, J. (deposition date: 2011-09-04, release date: 2011-12-14, Last modification date: 2024-11-06)

Primary citation

Patel, O.,Pellicci, D.G.,Uldrich, A.P.,Sullivan, L.C.,Bhati, M.,McKnight, M.,Richardson, S.K.,Howell, A.R.,Mallevaey, T.,Zhang, J.,Bedel, R.,Besra, G.S.,Brooks, A.G.,Kjer-Nielsen, L.,McCluskey, J.,Porcelli, S.A.,Gapin, L.,Rossjohn, J.,Godfrey, D.I.
Vbeta2 natural killer T cell antigen receptor-mediated recognition of CD1d-glycolipid antigen.
Proc.Natl.Acad.Sci.USA, 108:19007-19012, 2011

PubMed Abstract: Natural killer T cell antigen receptors (NKT TCRs) recognize lipid-based antigens (Ags) presented by CD1d. Although the TCR α-chain is invariant, NKT TCR Vβ exhibits greater diversity, with one (Vβ11) and three (Vβ8, Vβ7, and Vβ2) Vβ chains in humans and mice, respectively. With the exception of the Vβ2 NKT TCR, NKT TCRs possess canonical tyrosine residues within complementarity determining region (CDR) 2β that are critical for CD1d binding. Thus, how Vβ2 NKT TCR docks with CD1d-Ag was unclear. Despite the absence of the CDR2β-encoded tyrosine residues, we show that the Vβ2 NKT TCR engaged CD1d-Ag in a similar manner and with a comparable affinity and energetic footprint to the manner observed for the Vβ8.2 and Vβ7 NKT TCRs. Accordingly, the germline-encoded regions of the TCR β-chain do not exclusively dictate the innate NKT TCR-CD1d-Ag docking mode. Nevertheless, clear fine specificity differences for the CD1d-Ag existed between the Vβ2 NKT TCR and the Vβ8.2 and Vβ7 NKT TCRs, with the Vβ2 NKT TCR exhibiting greater sensitivity to modifications to the glycolipid Ag. Furthermore, within the Vβ2 NKT TCR-CD1d-αGalCer complex, the CDR2β loop mediated fewer contacts with CD1d, whereas the CDR1β and CDR3β loops contacted CD1d to a much greater extent compared with most Vβ11, Vβ8.2, and Vβ7 NKT TCRs. Accordingly, there is a greater interplay between the germline- and nongermline-encoded loops within the TCR β-chain of the Vβ2 NKT TCR that enables CD1d-Ag ligation.

PubMed: 22065767
DOI: 10.1073/pnas.1109066108

Experimental method

X-RAY DIFFRACTION (3.1 Å)