8D5N

Crystal structure of Ld-HF10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	121.503, 139.816, 87.344
Unit cell angles	90.00, 130.82, 90.00

Refinement procedure

Resolution	50.005 - 1.800
Rwork	0.178
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ld9
RMSD bond length	0.017
RMSD bond angle	2.080
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	77.000	1.864
High resolution limit [Å]	1.800	1.800
Rmerge		0.738
Rpim	0.050
Number of reflections	102152	17831
<I/σ(I)>	9.6
Completeness [%]	99.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	20% w/v PEG10K, 0.1M Sodium citrate tribasic dihydrate pH5.5, and 1M Lithium sulfate monohydrate