8D5N
Crystal structure of Ld-HF10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 121.503, 139.816, 87.344 |
Unit cell angles | 90.00, 130.82, 90.00 |
Refinement procedure
Resolution | 50.005 - 1.800 |
Rwork | 0.178 |
R-free | 0.21860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ld9 |
RMSD bond length | 0.017 |
RMSD bond angle | 2.080 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.000 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.738 | |
Rpim | 0.050 | |
Number of reflections | 102152 | 17831 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 99.6 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 20% w/v PEG10K, 0.1M Sodium citrate tribasic dihydrate pH5.5, and 1M Lithium sulfate monohydrate |