8D4V
Crystal Structure of Cathepsin G Inhibited by Eap2 from S. aureus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 77.561, 80.273, 99.761 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
Rmerge | 0.242 | 0.245 | 1.946 |
Rmeas | 0.253 | 0.257 | 2.094 |
Rpim | 0.075 | 0.076 | 0.759 |
Total number of observations | 544796 | ||
Number of reflections | 53684 | 5641 | 5297 |
<I/σ(I)> | 7.9 | ||
Completeness [%] | 99.6 | 99.2 | 99.6 |
Redundancy | 10.1 | 11 | 6.8 |
CC(1/2) | 0.968 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M BisTris (pH 6.5), 0.2M Ammonium Sulfate, 25%(w/v) PEG-3350 |