8D31

[AG/TC] Self-Assembled 3D DNA Cubic Tensegrity Triangle with 24 bp Arm Length

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-16
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	P 41 3 2
Unit cell lengths	187.499, 187.499, 187.499
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.190 - 7.450
R-factor	0.2756
Rwork	0.274
R-free	0.31620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gbi
RMSD bond length	0.007
RMSD bond angle	1.029
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.190	8.000
High resolution limit [Å]	7.450	7.450
Number of reflections	1558	217
<I/σ(I)>	14.5
Completeness [%]	92.8
Redundancy	70.7
CC(1/2)	0.998	0.606

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.75 M ammonium sulfate, 120 mM Tris, 120 mM Acetic Acid, 6 mM EDTA